Catalogue Number: AB04076-24.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CYP1A1; Cytochrome P450 1A1; Cytochrome P450 1A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Goat |
| Clone: | CRC4 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with synthetic zebrafish CYP1A peptide (VMEHYDTFDKDNIRDITDSLINHC) conjugated to KLH at the C-terminus cysteine. The resulting hybridomas were re-screened by ELISA against the core sequence (IRDITDSLI) to obtain this antibody. |
| Application: | ELISA, WB, IHC |
Q8UW07
140634
Purified
cyp1a
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
The binding reactivity of this antibody for synthetic peptide containing amino acid sequence 'IRDITDSLI' was determined using ELISA. The binding reactivity of this antibody towards CYP1A protein zebrafish liver cells, Poeciliopsis lucida hepatoma cell line, β-NF induced rainbow tout microsomes, mouse hepatoma cell line, rat liver, frog XLK-WG cell line and recombinant CYP1A1 expressed in HEK cells was determined using western blot. This antibody works well in formalin-fixed and paraffin-embedded liver tissues from various vertebrates for immunohistochemistry, as well as whole-mounted zebrafish embryos (PMID: 35878309).
This chimeric goat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.