Anti-Fibronectin EIIIB [NJB2]

Catalogue Number: AB04079-34.11-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	FN-EIIIB; EDB domain of fibronectin
Shipping Condition:	Blue Ice
Unit(s):	500 ug
Host name:	Camelid
Clone:	NJB2
Isotype:
Immunogen:	The original antibody was isolated from an alpaca-derived phage display library, by panning against the EIIIB domain of fibronectin.
Application:	ELISA, IF, IHC, IB, InVivoA

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This nanobody was shown to detect primary tumors and metastatic sites in vivo by specifically binding to the ECM with excellent specificity in multiple models of breast cancer, including human and mouse triple-negative breast cancer, and in melanoma, as measured by PET/CT imaging with 64Cu-NJB2. This nanobody recognized EIIIB-His by ELISA and in FN-containing EIIIB immunohistochemistry (IHC). Furthermore, its binding affinity to immobilized single EIIIB domain and a large recombinant rat FN fragment comprising FNIII7-15, including EIIIB (FN 7-15 EIIIB), was 1.9 x 10-9 M and 2.25 x 10-9 M, respectively, as determined by biolayer interferometry (BLI) (Jailkhani et al., 2019; PMID: 31068469). This nanobody was used for IHC of B16F10 and MC38 tumors to detect EIIIB expression (Xie et al., 2019; PMID: 30936321).

AB04079-34.11-BT Datasheet

Price

£2,215.00

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