Anti-GFP [GBP1 (Enh)]

Catalogue Number: AB04081-34.11-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:Green fluorescent protein; GFP Binding Protein 1; Enhancer
Shipping Condition:Blue Ice
Unit(s): 500 ug
Host name: Camelid
Clone: GBP1 (Enh)
Isotype:
Immunogen: The original antibody was generated by immunizing an alpaca with GFP.
Application: IF, InVitroA, InVivoA

Additional Text

Uniprot ID

P42212

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The Enhancer (Enh) anti-GFP nanobody, also known as GFP binding protein 1 (GBP1), was specifically used for manipulating protein conformation in vitro and in vivo; this nanobody caused a fourfold fluorescence enhancement by stabilizing the deprotonated, negatively charged state of the GFP chromophore, resulting in increased fluorescence intensity (Kirchhofer et al., 2010; PMID: 20010839).