Anti-Cathepsin B [2A2]

Catalogue Number: AB04088-10.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Cat B; CTSB; CPSB; APPS; APP secretase; Cathepsin B1
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	2A2
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing BALB/c mice with highly purified recombinant human cathepsin B expressed in E. coli.
Application:	ELISA, WB, Inh

Overview
Datasheets

Additional Text

Gene ID

1508

Uniprot ID

P07858

Gene Name

CTSB

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody is capable of binding cathepsin B in an antigen-immobilized ELISA. The binding reactivity of this antibody to cathepsin B was also confirmed using Western blot. This antibody significantly inhibited the invasion of ras-transformed MCF-10A neoT cells through matrigel. This antibody was further reported to inhibit cathepsin B activity against BODYPY FL casein significantly (Fan et al., 2002; PMID: 12530548). In a study, the intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using this antibody. This antibody was shown to impair both the intracellular and the extracellular fractions of cathepsin B activity (Premzl et al., 2003; PMID: 12581740). This antibody was also used in the generation of an ammodytoxin-anti-cathepsin B immuno-conjugate to deliver secretory phospholipase A2 into cancerous cells (Premzl et al., 2008; PMID: 18221975). This antibody was reported to potentiate cathepsin B exopeptidase activity with an activation constant (Ka) of 22.3 nm, although simultaneously inhibiting its endopeptidase activity (Mirkovi et al., 2009; PMID: 19656187).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB04088-10.0-BT Datasheet

Price

£1,825.00

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