Anti-STAT3 phosphorylated [AbAb1-pSTAT3]
Catalogue Number: AB04100-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | APRF; Signal transducer and activator of transcription 3; Acute-phase response factor |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | AbAb1-pSTAT3 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide pSTAT3 (PGSAAP-pY-LKTKGGGSC) conjugated with KLH as an immunogen. |
| Application: | ELISA, IP, WB |
Additional Text
Gene Name
STAT3
Gene ID
6774
Uniprot ID
P40763
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding reactivity of this antibody to recombinant phosphorylated pSTAT3 peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of STAT3 protein IFN-α-treated cells. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pSTAT3 in the cell lysate of HeLa cells treated with IFN-α (US20090233358).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
AB04100-10.0-BT Datasheet
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