Anti-Viral hemorrhagic septicemia virus [3F1A2]

Catalogue Number: AB04106-21.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	G; Spike glycoprotein
Shipping Condition:	Blue Ice
Unit(s):	0.05 mg
Host name:	Mouse
Clone:	3F1A2
Isotype:	IgM
Immunogen:	The original antibody was generated by immunizing BALB/c mice with Egtved virus by intraperitoneal injections five times over two months, followed by an additional intravenous booster injection on week nine. The hybridoma cell line secreting clone 3F1A2 was selected from a subsequent fusion after three more months of continued immunization.
Application:	ELISA, NT, InVitroA

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

UniProt ID

P27662

Application Notes

The neutralizing activity of the original format of this antibody (mouse IgG1) against different VHSV isolates was determined by 50% PNT; it neutralized type I isolates VHSV (I-F1 and I-92) and type II isolate VHSV (II-31) well, but higher concentrations were needed to neutralize type III isolates (III-51 and III-37). ELISA was used to detect and quantify the antibody's binding efficiency to various VHSV strains. The antibody's binding kinetics were evaluated by surface plasmon resonance (SPR) analysis resulting in a KD of 2.4 nM for the immobilized G protein of VHSV strain I-F1 and a KD of 12.1 nM for the immobilized G protein of VHSV strain I-92 (Lorenzen et al., 2000; PMID: 10938729). The Fab and recombinant single-chain antibody fragments (scAbs) versions of this antibody neutralized VHSV, indicating that the Fc moiety and divalency of the antibody molecules are unnecessary for neutralization. However, the neutralizing activity of Fab and scAb fragments was generally lower compared to the parent antibody due to the higher avidity of the divalent parent molecules; the Fab and scAb fragments exhibited a 10-100-fold increase in kd compared to the parent MAb, while the kas were similar. The increased kd was accompanied by decreased neutralizing activity. The KD measured against immobilized G protein of VHSV strain DK-F1 was 2.4 nM for the parent MAb and 227 and 77 nM for the daughter Fab and scAb, respectively (Cupit et al., 2001; PMID: 11682124).

Short Description

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB04106-21.0 Datasheet

Price

£525.00

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