Anti-IgG [RF61]
Catalogue Number: AB04107-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Monoclonal Secondary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | RF61 |
| Isotype: | IgG |
| Immunogen: | The original antibody was produced by an Epstein Barr virus (EBV)-transformed B-cell line prepared from peripheral CD5+ blood lymphocytes of a Gm(a-), (x-), (b+), (g-), (f+) patient with classical rheumatoid arthritis (RA). |
| Application: | ELISA, HA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Specificity
IgG1; IgG2; IgG3; IgG4; Immunoglobulin G; Immunoglobulin gamma-1; Immunoglobulin G subclass 1; Immunoglobulin gamma-2; Immunoglobulin G subclass 2; Immunoglobulin gamma-3; Immunoglobulin G subclass 3; Immunoglobulin gamma-4; Immunoglobulin G subclass 4; 274.RA.F4; RF mAb 61; mAb 61
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
UniProt ID
P0DOX5
Application Notes
The binding activity of this antibody's original format (human IgM) to IgG Fc fragments was measured by ELISA, and a dissociation constant (Kd) of 6.0x10-7 M was calculated (Burastero et al., 1988; PMID: 3264319). This antibody exhibited strong reactivity with both monoclonal and polyclonal human anti-Rh IgG antibodies bearing the Gm(a) or Gm(g) specificity in hemagglutination assays (HAs), displaying a higher affinity for Gm(g). Additionally, it demonstrated efficient binding to heat-altered autologous Gm(a-), (g-) IgG and exhibited heteroclitic specificity at the clonal level in an autologous system (Williams Jr et al., 1992; PMID: 1380541). The binding of this antibody's original format to human IgG subclasses as measured by ELISA demonstrated that its binding specificity is IgG1~IgG2~IgG3>IgG4 (Duquerroy et al., 2007; PMID: 17395205).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
AB04107-23.0-BT Datasheet
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