Anti-1,3-diketone hapten [33F12]
Catalogue Number: AB04118-2.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Hapten 1,3-diketone; β-keto acids; beta keto acids |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 33F12 |
| Isotype: | IgG2a |
| Immunogen: | The original antibody was generated by immunizing GIX+ mice with a keyhole limpet hemocyanin (KLH)-coupled diketone hapten. |
| Application: | Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody efficiently catalyzes aldol and retro-aldol reactions and covalently binds 1,3-diketone hapten. The (kcat/KM)/kuncat is approximately 109 at pH 7.5 as measured by the consumption of substrates and production of aldol products (monitored using reversed-phase high-performance liquid chromatography (HPLC) and enzymatic assays) (Wagner et al., 1995; PMID: 8525368). This antibody efficiently catalyzed the decarboxylation of two hapten-related β-keto acids (marked 4 and 5 in scheme 1 in the publication) with significant rate enhancements (kcat/kuncat) of 4959 and 11,261, respectively (Bjoernestedt et al., 1996). The crystallization of the Fab version of this antibody revealed a binding pocket characterized by a narrow, elongated cleft, where the catalytic lysine residue is prominently located (Barbas III et al., 1997; PMID: 9405338).
AB04118-2.0-BT Datasheet
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