Anti-HER1 [048-006]
Catalogue Number: AB04150-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Epidermal growth factor receptor; EC:2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | 048-006 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was isolated by panning a phage display library against HER1. |
| Application: | WB, InVivoA, SPR, ADCC |
Additional Text
Gene ID
1956
Gene Name
EGFR
Uniprot ID
P00533
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody was able to inhibit the phosphorylation signal of HER1 in three kinds of cells (renal cell carcinoma (CCF-RC1, Caki-1) and epidermoid cancer (A431)) in a concentration-dependent manner, as shown by western blot analysis. The antibody could inhibit the binding to EGF, using A431 cells. The binding affinity of the original format of the antibody to HER1 was measured by surface plasmon resonance (Kd= 0.48 pM). The IgG1 format of the antibody exhibited ADCC activity in the cell line in which HER1 is expressed (CCF-RC-1, NCI-H1373, HT-29, ACHN and A-431 cell lines). The IgG1 format of the antibody was used in in vivo antitumor experiments. Human lung cancer cells H1373 cells or epidermoid tumor A-431 were transplanted into a mouse so as to produce a tumor and, the antibody was administrated. The antibody significantly reduced the tumor volume compared to the control group. The effect was comparable to ERBITUX when human lung cells H1373 were used (US9388249B2).
Short Description
This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
AB04150-10.0-BT Datasheet
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