Anti-Chikungunya virus [CAP5A-F6]
Catalogue Number: AB04153-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CHIKV; IM-CKV065 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | CAP5A-F6 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated from a phage Fab library created from the B-cells of CHIKV infected patients. The library was panned against retroviral (Gag core) and CHIKV (alphavirus capsid core) virus-like particles (VLPs) displaying the native form of CHIKV E2/E1. |
| Application: | FC, NT, SPR, PRNT |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding affinity of the original format of the antibody to CHIKV VLPs was measured by surface plasmon resonance (Kd= 9.3 nM). The original antibody demonstrated high reactivity with E2/E1 presented on cells, as shown by flow cytometry. The original format of the antibody was tested in a cellular neutralization assay against CHIKVL, where showed an average IC50 of 170 ng/ml. The original antibody was tested for antiviral activity using native replication-competent CHIKV viruses. In a plaque reduction neutralization test assay, the antibody could neutralize CHIKV strain 37997 with a PRNT50 value of 81 ng/ml (US9902765B2).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB04153-23.0-BT Datasheet
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