Anti-gelatinase B [REGA-3G12]

Catalogue Number: AB04197-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	0.1 mg
Host name:	Rabbit
Clone:	REGA-3G12
Isotype:	IgG
Immunogen:	The original antibody was generated by immunizing Balb/c mice with a purified gelatinase B enzyme preparation.
Application:	ELISA, IP, WB, Inh, IB

Overview
Datasheets

Additional Text

Gene Name

MMP9

Gene ID

4318

Uniprot ID

P14780

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Specificity

GB; GELB; MMP 9; MMP9; MMP-9; gelatinase MMP 9; 92-kDa gelatinase; matrix metalloproteinase 9; Matrix metalloproteinase-9; neutrophil gelatinase; natural human neutrophil gelatinase B; type V collagenase; 92-kDa type IV collagenase; macrophage gelatinase; 95 kDa type IV collagenase/gelatinase; collagenase IV; collagenase type IV; type IV collagen metalloproteinase; 3G12; 3G12T; 3G12 scFv; 3G12scFv

Application Notes

The specificity of this antibody's original format (mouse IgG1, κ) was evaluated by ELISA; it specifically bound to gelatinase B with a binding affinity of 2.1 x 10-9 M. This antibody detected both native and H2NPhAcHg-treated gelatinase B in an immunoblot, both in non-reducing and reducing conditions. Furthermore, this antibody was found to inhibit gelatinase B activity in vitro (Paemen et al., 1995; PMID: 8575432). The binding of the scFv version of this antibody to gelatinase B was confirmed in an ELISA. The original antibody and its F(ab')2 and scFv derivatives inhibited gelatinase B in vitro (Zhou et al., 1997; PMID: 9323036). The following scFv derivatives of this antibody were evaluated for their inhibitory effect toward gelatinase B: REGA-3G12 scFv with (His6) monomer (10 µM), REGA-3G12 scFv with (His6) dimer (5 µM), and REGA-3G12 scFv without a tag (1.25, 2.5, and 5 µM). They achieved 6.5%, 65.9%, 8.8%, 32.4%, and 44.4% inhibitory activity, respectively. Moreover, the scFv His-tag dimer variant was found to cross-react with gelatinase A with a 75% inhibitory activity (same antibody concentration) (Hu et al., 2004; PMID: 15104254). The original format of the antibody was used to detect and characterize the binding of this antibody to gelatinase B via immunoprecipitation (IP) and Western blot (WB), and the catalytic domain in the aminoterminal segment of gelatinase B was identified as its target (Martens et al., 2007; PMID: 17137715).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB04197-23.0 Datasheet

Price

£430.00

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