Anti-RSPO1 [89M5]
Catalogue Number: AB04198-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | R-spondin-1; Roof plate-specific spondin-1; hRspo1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Rabbit |
| Clone: | 89M5 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing mice against recombinant human RSPO1 protein amino acids 31-263. |
| Application: | FACS, FC, InVivoA, SPR |
Additional Text
Gene ID
284654
Uniprot ID
Q2MKA7
Gene Name
RSPO1
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding affinity of the original format of the antibody to human and mouse RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM respectively). Further, the binding affinity of the humanized version of the antibody, h89M5-H2L2, to human RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM). The original format of the antibody could inhibit tumor cell growth in vivo in an ovarian tumor xenograft model as single agents and in combination with a chemotherapeutic agent. The original format of the antibody could inhibit tumor cell growth in vivo in a pancreatic tumor xenograft model in combination with a chemotherapeutic agent. The original antibody was able to block RSPO1-induced β-catenin signaling in HEK-293 cells. The original antibody blocked binding of RSPO1 to LGR5 in HEK-293 cells, as measured by FACS. The original and humanized format of the antibody, could bind to human RSPO1 expressed on HEK-293 cells, as shown by FACS analysis (US8802097B2).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB04198-23.0 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations