Anti-Tenuazonic acid [3F9]

Catalogue Number: AB04211-10.159-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Nb-3F9; TeA; Alternaria mycotoxin tenuazonic acid; (5S)-3-Acetyl-5-[(2S)-butan-2-yl]-4-hydroxy-1,5-dihydro-2H-pyrrol-2-one
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Human
Clone:	3F9
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing a male Bactrian camel with TeA-CMO-KLH followed by antibody library construction and phage display-based selection.
Application:	ELISA, IA

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The activity and sensitivity of the original format of this antibody (VHH) were assessed by ic-ELISA. Nb-3F9-Nluc, a biofunctional nanobody fusion, was generated and its nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity were analyzed. The performance of Nb-3F9-Nluc and the original Nb-3F9 was evaluated using two-step ELISA with HRP-anti-6xHis tag secondary antibody. The IC50 value of ELISA based on Nb-3F9-Nluc was 80.3 ng/mL, and that for Nb-3F9 was 89.5 ng/mL. Additionally, Nb-3F9 and Nb-3F9-Nluc were used, respectively, in the development and optimization of chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay (Wang et al., 2020; PMID: 32702970). TeA is a tetrameric acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control.

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Dromedary VHH format, for improved compatibility with existing reagents, assays and techniques.

AB04211-10.159 Datasheet

Price

£430.00

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