Anti-KRAS G12V [G12V-34]
Catalogue Number: AB04234-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Rabbit |
| Clone: | G12V-34 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated through the construction of a phage-displayed single-chain fragment variable (scFv) library from mice splenocytes immunized with specific mimotope peptides. The library was subjected to three rounds of biopanning on G12V and G13D peptide-coated plates. |
| Application: | ELISA, IF, CTA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity
Mutated KRAS G12V; Mutated Kirsten rat sarcoma viral oncogene homolog G12V; Kirsten Rat Sarcoma virus; Kirsten Rat Sarcoma virus; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2; K-ras; KRAS proto-oncogene; GTPase; c-Ki-ras2; OES; c-Ki-ras; K-Ras 2; 'C-K-RAS; K-Ras
Application Notes
The original format of this antibody (scFv) was screened against WT and mutated G12 and G13 KRAS and shown to specifically bind mKRAS G12V in an ELISA. The scFv format of this antibody was fused to mHALT-1 toxin and exhibited cytotoxic effects on SW-480 colorectal cancer cells with IC50 of 25.39 µg/mL, with minimal cytotoxicity effect on NHDF cells. The scFv format of this antibody was fused to enhanced GFP and used for IF (Teo et al., 2021; PMID: 33959410).
Short Description
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
AB04234-23.0 Datasheet
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