Anti-HLAII-DP [CBL 100 BRA FB6]

Catalogue Number: AB04281-10.0-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:HLA-DPB1; HLA-DP1B; MHC class II antigen DPB1; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA class II histocompatibility antigen, DP beta 1 chain; MHC II; MHCII; BRA-FB6; BRAFB6
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Human
Clone: CBL 100 BRA FB6
Isotype: IgG1
Immunogen: The original antibody was raised by immunizing a BALB/c mouse with a non-T, non-B human acute lymphoblastic leukemia REH6 cell line.
Application: ELISA, FC, IP, InVitroA

Additional Text

Gene Name

HLA-DPB1

Gene ID

3115

Uniprot ID

P04440

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original version of this antibody was used to immunoprecipitate HLAII-DP from the detergent lysate of radioiodinated REH6 cells (Horeji et al., 1988; PMID:3187993). This antibody was used for immunofluorescence on B-lymphoblastoid, lymphoma and non-T, non-B leukemia cell lines (Polakova et al., 1985; PMID:3911080). This antibody was used for an ELISPOT assay on CD4+ T cells co-cultured with various stimulators (Demachi-Okamura et al., 2008; PMID:18754877). This antibody was used for flow cytometry on Hepatocytes (Guo et al., 2003; PMID:12717860), and on human PBMCs (Haylett et al., 2009; PMID:19544309). This antibody was also used in vitro on Raji cells to induce ERK1/2 activation (Haylett et al., 2009; PMID:19544309).