Catalogue Number: AB04318-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | 121 SLE; sialyl Lewis a |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | 121SLE |
| Isotype: | IgG |
| Immunogen: | The original antibody was raised by immunizing a mouse with ten precipitin lines obtained after immunodiffusion using monoclonal antibody NS 19-9 and mucins isolated from an ovarian cyst of a OLe (a+b-) patient. |
| Application: | ELISA, FC, ICC, IHC |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
The original version of this antibody was used for immunohistochemistry on the normal gastrointestinal mucosa of Le-positive individuals (Herrero-Zabaleta et al., 1987; PMID:2444292), and on endometrial tissue specimens obtained in different phases of the menstrual cycle (Hey and Aplin., 1996; PMID:8910004). This antibody was used for an ELISA on carbohydrate proteins (Manimala et al., 2007; PMID:17483136), and on MUC1 samples (Hey and Aplin., 1996; PMID:8910004). Furthermore this antibody was used for immunocytochemistry on HT 29 tumour cells (Koehler et al., 2009; PMID:20010946). The original version of this antibody was used for flow cytometry on ovarian cancer cell line SW626 and SCLC cell line DMS79 (Ragupathi et al., 2009; PMID:19190907).
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.