Anti-MUC-1 [VU-4H5 ]
Catalogue Number: AB04350-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Human |
| Clone: | VU-4H5 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing mice with a synthetic glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3-COOH, conjugated to bovine serum albumin. |
| Application: | FC, IF, WB, IHC |
Additional Text
Gene Name
MUC1
Gene ID
4582
Uniprot ID
P15941
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Short Description
This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Application Notes
This antibody was used for the flow cytometric analysis of mucin-1 core protein expressed on human multiple myeloma (ARH77, OCI My-5, RPMI 8226, and S6B45), breast cancer (MCF.7 and ZR-75), and ovarian cancer (OVC.3 and 36M) cell lines. This antibody was also used in the phenotypic analysis of cancer patients PBMC cells using immunofluorescence (PMID: 9949172). This antibody was used in the western blot analysis of cell lysates obtained from human MM (S6B45 and RPMI 8226) and prostate cancer (DU145) (PMID: 12090474). This antibody was also used to staining of mucin-1 peptide core uncovered due to aberrantly O-glycosylated tandem repeat region of tumor MUC1 (PMID: 12090474). This antibody was also used in immunohistochemical study of co-expression of hypoglycosylated MUC1 with p-p65 and EzH2 in colon adenocarcinoma (PMID: 29285251).
Specificity
Mucin-1; CD227; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; VU4H5
AB04350-10.3 Datasheet
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