Anti-CD4 [3F11]
Catalogue Number: AB04387-34.11-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | VHH Nanobodies |
| Alias: | CD4mut; CD4 molecule; OKT4D; IMD79; 03F11; CD4#8; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 500 ug |
| Host name: | Camelid |
| Clone: | 3F11 |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA, FACS, FC, Blk |
Additional Text
Gene Name
CD4
Gene ID
920
Uniprot ID
P01730
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding of the original version of this antibody (VHH) to CD4 was characterized in an ELISA. In a FACS binding assay, this antibody exhibited an EC50 of 0.7 nM, 1.0 nM, and 0.76 nM for MOLM-13, THP-1, and T cells, respectively, and in an HIV-1 neutralization it exhibited an IC50 of 29.3 nM for PMBCs + NL4.3. This antibody was used to construct two bi-specific nanobodies with clone 281F12 (Ab04369 Anti-CXCR4), one where it was in the 1st position and one where it was in the 2nd position. Alone, it bound CXCR4+/CD4+ MOLM-13 cells with a binding EC50 of 0.6 nM and CXCR4+/CD4low Jurkat E6-1 cells with a binding EC50 of 61 nM. Its bi-specific constructs bound the CXCR4+/CD4+ MOLM-13 and CXCR4+/CD4low Jurkat E6-1 cells with a binding EC50 of 1.1 and 11 nM when it was in the 2nd position and 0.7 and 1.1 nM when it was in the 1st position. Furthermore, its bi-specific constructs exhibited a chemotaxis IC50 of 0.59 and 110 nM on CXCR4+/CD4+ MOLM-13 and CXCR4+/CD4low Jurkat E6-1 cells when it was in the 2nd position, and 1.29 and 460 nM when it was in the 1st position. This nanobody was effective at blocking CXCR4-tropic HIV1 strain NL4.3 and the CCR5-tropic BaL, and its bi-specific construction was extremely potent in inhibiting HIV-1 X4 virus replication, especially when evaluated in PHA-stimulated PBMCs. This antibody was also used to construct bi-specific nanobodies with Ab04390 and Ab04391 (anti-IL23R [150D02] and [150H07]) and Ab04392 and Ab04393 (Anti-IL12RB1 [148C09] and [148F09]). In these bi-specific constructions, it served as an anchor (US20220242958).
AB04387-34.11-BT Datasheet
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