Anti-Cap, Capsid protein [3A5]
Catalogue Number: AB04396-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | ORF2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | 3A5 |
| Isotype: | IgG |
| Immunogen: | The original antibody was raised by immunizing a BALB/c mouse with a recombinant capsid protein of PCV2 expressed by a baculovirus vector |
| Application: | ELISA, IF, IHC, NT, InVitroA |
Additional Text
Purification
Purified
UniProt ID
O56129
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original antibody was used for an ELISA on PCV2-positive serum (Huang et al., 2011; PMID:20923690), on PCV2a/LG-infected PK15 cells cultures (Huang et al., 2019; PMID:30863876), and on purified PCV2 (Bian et al., 2021; PMID:34959519). Further this antibody was used for immunohistochemistry on inguinal lymph nodes of PCV2-inoculated pigs (Huang et al., 2016; PMID:27020283) (Huang et al., 2019; PMID:30863876). This antibody was used for an neutralization assay on PCV2a/LG. PCV2a/LG was incubated with this antibody after which the mixture was added to PK15 cells (Huang et al., 2020; PMID:32075932) (Yu et al., 2022; PMID:35945364). This antibody was also used for immunofluorescence to test the absorption of PCV2 to PK15 cells. The structure of the original antibody was determined using cryo-EM (Huang et al., 2020; PMID:32075932). This antibody was used for purification of proteins from PCV2a/LG (Bian et al., 2021; PMID:34959519)
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB04396-23.0 Datasheet
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