Anti-Outer capsid protein VP4 [#9]

Catalogue Number: AB04397-23.0-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:VP4; Hemagglutinin; scFv9; Spike protein VP4
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Rabbit
Clone: #9
Isotype: IgG
Immunogen: The original antibody was raised from intestinal B cells isolated from adults undergoing bariatric surgery for obesity.
Application: ELISA, IF, NT, Crstapy

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The original antibody was used for an ELISA on virus-like particles, bacterially expressed VP8 and VP5 (Nair et al., 2017; PMID:28637924). Further this antibody was used for a neutralization assay by using the antibody virus mixture on MA104 cells (Nair et al., 2017; PMID:28637924), and on MA104, HT-29, CaCo-2, CV-1, and HEK293 cells (Feng et al., 2019; PMID:31403468). This antibody was used for immunofluorescence on RV-infected MA104 cells (Nair et al., 2017; PMID:28637924). The structure of this antibody was determined using x-ray crystallography (Feng et al., 2019; PMID:31403468).

Short Description

This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Uniprot ID

P11193