Anti-Bordetella pertussis lipooligosaccharide [HBp2]

Catalogue Number: AB04459-15.0-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Shipping Condition:Blue Ice
Unit(s): 500 ug
Host name: Human
Clone: HBp2
Isotype: IgM
Immunogen: The original antibody was raised from splenic lymphocytes isolated from fresh human spleen tissue, that were immunized in vitro against Bordetella pertussis lipooligosaccharide.
Application: ELISA, WB, RIA, NT

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The original antibody was used for a dot blot on different bacteria lysates. This antibody was used for a radioimmunoassay on live bacteria, this antibody bound to surface-exposed epitopes on B. pertussis strains 9340 and Tohama 1, but not other strains. The biological activity of this antibody against B. pertussis and B. bronchiseptica was assessed. It showed a high degree of killing on B. pertussis strain 9340. This antibody was used for an ELISA on B. pertussis OMP preparations (Brodeur et al., 1991; PMID:1721552).