Catalogue Number: AB04459-15.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Human |
| Clone: | HBp2 |
| Isotype: | IgM |
| Immunogen: | The original antibody was raised from splenic lymphocytes isolated from fresh human spleen tissue, that were immunized in vitro against Bordetella pertussis lipooligosaccharide. |
| Application: | ELISA, WB, RIA, NT |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
The original antibody was used for a dot blot on different bacteria lysates. This antibody was used for a radioimmunoassay on live bacteria, this antibody bound to surface-exposed epitopes on B. pertussis strains 9340 and Tohama 1, but not other strains. The biological activity of this antibody against B. pertussis and B. bronchiseptica was assessed. It showed a high degree of killing on B. pertussis strain 9340. This antibody was used for an ELISA on B. pertussis OMP preparations (Brodeur et al., 1991; PMID:1721552).