Anti-CD11a [AL-57]
Catalogue Number: AB04500-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | LFA-1A; integrin LFA-1; Integrin alpha-L; activated LFA-1 clone 57; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain ;Leukocyte function-associated molecule 1 alpha chain |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | AL-57 |
| Isotype: | IgG |
| Immunogen: | The original antibody was selected from a human Fab-displaying library against the HA open conformation of the LFA-1 I domain. |
| Application: | ELISA, FC, SPR, Crstion |
Additional Text
Gene Name
Itgal
Gene ID
3683
Uniprot ID
P20701
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The cell-binding specificity of the original and IgG1 format of the antibody was determined using flow cytometric analysis with HA and LA cells, the antibody bound to HA cells in a dose-dependent manner but did not bind to LA cells. The antibody preferentially binds to the active conformation of LFA-1 and blocks LFA-1-mediated adhesion and lymphocyte proliferation (Huang et al., 2006; PMID: 16888085). The original format of the antibody could discriminate among low-affinity, intermediate-affinity (IA), and HA states of LFA-1. Furthermore, the binding of the antibody depended on the presence of Mg2+. The antibody showed no binding to the WT I domain, intermediate binding to the IA I domain (KD = 4.7 µM), and good binding to the HA I domain by SPR (KD = 0.023 µM). The original format of the antibody showed affinity increases on a subset (~10%) of lymphocyte cell surface LFA-1 molecules upon stimulation with CXC chemokine ligand 12 (CXCL-12). The IgG1 format of the antibody could bind to HA I domain expressed on the surface of K562 cells presence of Mg2+, as tested by using immunofluorescent flow cytometry (Shimaoka et al., 2006; PMID: 16963559). The crystal structures of the Fab fragment of the antibody alone and in complex with IA were determined (Zhang et al., 2009; PMID: 19805116).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
AB04500-23.0-BT Datasheet
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