Anti-Capsid protein VP1 [Nano-85]
Catalogue Number: AB04580-34.11-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | VHH Nanobodies |
| Alias: | CP; Capsid protein; ABCD_AG267; ABCD_AH279 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Camelid |
| Clone: | Nano-85 |
| Isotype: | |
| Immunogen: | The original antibody was generated by immunizing a single alpaca with the GII.10 virus-like particle protein. |
| Application: | ELISA, EM, Inh, Crstapy |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
UniProt ID
Q5F4T5
Application Notes
The reactivity of the original antibody (alpaca VHH) against norovirus VLPs and P domains was determined using ELISA. It was shown to be reactive with antigenically distinct norovirus VLPs, including GII.4, GII.10, and GII.12. Using isothermal titration calorimetry, a Kd of 7 nM was measured against GII.10 P domain and a Kd of 3.5 nM against GII.4 P domain. The crystal structure of this antibody in complex with norovirus GII.10 P domain, Saga-2006 GII.4 P domain, and 2012 NSW GII.4 P domain were determined. This antibody can bind intact norovirus VLPs and virions and promotes their disassembly (Koromyslova & Hansman, 2015; PMID: 25520510). The epitope of this antibody was further characterized in X-ray crystallography and cryo-EM. This antibody's binding mechanism involves impairing capsid motions and integrity, which inhibits multiple stages of the Norovirus life cycle (Koromyslova & Hansman, 2017; PMID: 29095961).
AB04580-34.11 Datasheet
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