Anti-HMGB1 [2G7]
Catalogue Number: AB04610-6.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Hmg-1; Hmg1; Amphoterin; Heparin-binding protein p30; High mobility group protein 1; High mobility group protein B1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rat |
| Clone: | 2G7 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was raised by immunizing BALB/c mice with recombinant CBP-Rat HMGB1. |
| Application: | ELISA, IF, WB, InVivoA |
Additional Text
Gene Name
HMGB1
UniProt ID
P63159
Purification
Purified
Gene ID
25459
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric Rat antibody was made using the variable domain sequences of the original mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
The original antibody was used for an ELISA on recombinant rat HMGB1. This antibody was used for a western blot on recombinant rat HMGB1. The epitope of this antibody was determined using peptide binding. Mice were subjected to a cecal ligation and puncture, after which this antibody was administered. It showed an increased survival rate in these septic mice (US7632500B2). This antibody was used for immunofluorescence on RAW 264.7 cells, to study intracellular HMGB1. This antibody was used for an ELISPOT on RAW 264.7, C57 and HMC-12 cells (Waehaemaa et al., 2007; PMID:16980509). This antibody was administered to mice subjected to ACLT, the antibody was able to protect cartilage (Aulin et al., 2020; PMID:31982563). This antibody was tested in two murine arthritis models, it showed a decrease in general disease severity (Schierbeck et al., 2011; PMID:21666956). This antibody was used in vivo on SOD1G93A transgenic mice, to test the effect of this antibody on ALS, the antibody did not increase survival time but it did improve early motor deficits and reduced inflammation (Lee et al., 2019; PMID:30782181).
AB04610-6.1-BT Datasheet
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