Anti-IgG-Fc Domain [IgG-Fc-16]

Catalogue Number: AB04642-34.11-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:VHH Nanobodies
Shipping Condition:Blue Ice
Unit(s): 500 ug
Host name: Camelid
Clone: IgG-Fc-16
Isotype:
Immunogen: The original antibody was generated by immunizing llamas with mammalian IgG antibodies and Fc fragments, creating the appropriate phage display library, and panning against the original target.
Application: ELISA, SPR

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The reactivity of the original version of this antibody (camelid (llama) VHH) was tested using ELISA. Its binding affinity (KD) was measured using SPR and was found to be 13.1, 6.81, 16.2, 21.3, 1.70, 2.79, and 8.41 nM for human IgG, human IgG1, human IgG2, human IgG3, human IgG4, bovine IgG, and mouse IgG, respectively. Its binding to human, mouse, and bovine IgG was further verified via chromatography testing; the dynamic binding capacity of the antibody when immobilized onto NHS sepharose was 11.5 mg human IgG/ml matrix (US9040666B2).