Anti-GM1-bound Aβ [4396]

Catalogue Number: AB04720-10.3-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Gaβ; GM1 ganglioside-bound amyloid β-protein; 4396C
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Human
Clone:	4396
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing mice with purified human brain-derived GM1-Aβ.
Application:	IF, EM, WB, IM

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original mouse IgM antibody was developed and tested to specifically recognize Aβ bound to GM1 (GM1-Aβ). A subsequent version, 4396C, an IgG2a subtype chimeric antibody, was generated and evaluated. The chimeric antibody retained its specificity for GM1-Aβ, as demonstrated by electron microscopy (EM), where it specifically labeled GM1-containing liposomes incubated with Aβ; Western blotting (WB), which confirmed the antibody's specificity for GM1-Aβ; a Thioflavin T (ThT) assay, which showed that the antibody inhibited amyloid fibril formation in a dose-dependent manner when incubated with GM1-containing liposomes and Aβ; and immunoelectron microscopy (IM), where it effectively bound to newly formed amyloid fibrils in the presence of synthetic Aβ and GM1-containing liposomes (US7339035B2). The chimeric antibody was assessed in three polymerization systems: (1) polymerization initiated by adding Aβ fibril fragment, (2) Aβ40 polymerization initiated from GAβ40, and (3) Aβ42 polymerization initiated from GAβ42. It exhibited suppression of the Aβ extension (US7741448B2).

AB04720-10.3 Datasheet

Price

£430.00

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