Anti-tumor-associated gangliosides [GMA1]
Catalogue Number: AB04725-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | GD2; GD3; GM3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | GMA1 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was developed by fusing pooled lymph node lymphocytes from cancer patients with the human lymphoblastoid cell line, SHFP-1. |
| Application: | ELISA, FACS, FC, IF, EIA, TLC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of the original antibody (human IgG1) was evaluated using ELISA and TLC. It exhibited high specificity for major tumor-associated gangliosides GD3, GD2, and GM3, with minimal cross-reactivity observed with other gangliosides such as GD1b, GT1b, and GQ1b. GD3 demonstrated the highest binding affinity, while GM3 exhibited the lowest. Additionally, the antibody showed binding to melanoma (SK-MEL-28), stomach (KATO-III), pancreas (PANC-1), and neuroblastoma (U87MG) cell lines in an enzyme immunoassay (EIA). It reacted with both live and fixed SK-MEL-28 and PANC-1 cells in IF and FACS assays, indicating its ability to bind both cell surface and intracellular antigens. A recombinant version of the antibody was developed and shown to have similar reaction characteristics as the antibody produced by the hybridoma cell line, demonstrating that the specificity was not altered during molecular cloning (EP1056861A2) (Mukerjee et al., 1998; PMID: 9627053).
AB04725-10.0-BT Datasheet
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