Anti-H3K27me3 [2E12LI]
Catalogue Number: AB04736-1.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 2E12LI |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with a 20-mer peptide (KQLATKAAR (trimethyl K) SAPATGGVKC) containing histone H3K27 trimethylated lysine and conjugated with KLH. |
| Application: | IF, FA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody was used for the development of a H3K27me3-specific intracellular antibody or H3K27me3-mintbody, which was a fusion protein comprising variable domain regions of 2E12LI antibody fused to a fluorescent protein and expressed in mouse teratocarcinoma cell line MC12. This mintbody was capable of tracking H3K27me3 in living cells. Mouse MC12 cells, which stably express H3K27me3-mintbody (sfGFP) was used to localize H3K27me3-enriched chromatin in living cells. The binding specificity of bacterially expressed and purified 2E12LI-sfGFP in vitro was evaluated using a modified histone peptide array. (PMID: 33605056).
Specificity
Trimethylated Histone H3 Lys27; trimethyl-Histone H3 (Lys27); histone H3; H3K27; Trimethylated histone H3; Histone H3 trimethylated at Lysine 27; nucleosome; H3K27ME3; post translational modification; histone post-translational modification; 2E12 mutant M86L/M158I; 2E12
AB04736-1.1 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations