Anti-CA 19-9 [1116-NS-19-9]
Catalogue Number: AB04810-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD43; Colorectal carcinoma; CA19-9; sialyl Lewis A; sLe A; 1116-56-2; 1116NS19.9; 1116-NS-19-9 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | 1116-NS-19-9 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with human colorectal carcinoma cell line SW 1116. |
| Application: | FC, IF, RIA, IHC, SPR, Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
The original version of this antibody was used in a radioimmunoassay (RIA), a mixed Hemadsorption Assay (MHA), and an immunofluorescence (IF) assays to validate its specificity for human colorectal carcinoma cells. It was also tested on fresh tumor tissue from colorectal carcinoma patients, where it was found to bind selectively to tumor cells, but not normal intestinal tissue, further confirming its specificity for colorectal carcinoma (Herlyn et al., 1979; PMID: 286328). This antibody was crystallized with and without its target antigen. Furthermore, in a surface plasmon resonance (SPR) assay a KD of 14.7 µM was measured, and the antigen recognition of an scFv version of this antibody was measured via flow cytometry (FC) (Borenstein-Katz et al., 2021; PMID: 34119488). The use of this antibody in IHC was successfully demonstrated in a doctoral dissertation (Khilji, 2023).
AB04810-23.0 Datasheet
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