Anti-CXCR3 [53Hu37]
Catalogue Number: AB04813-10.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD183; CXC-R3; CXCR-3; CKR-L2; IP-10 receptor; GPR9; C-X-C chemokine receptor type 3; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; clone 53.6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Human |
| Clone: | 53Hu37 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by humanizing antibody clone "53". |
| Application: | CTA, Depl, InVivoA, SPR, Ant |
Additional Text
Gene ID
2833
Uniprot ID
P49682
Gene Name
CXCR3
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This human IgG1 antibody was evaluated for its effector functions, including Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) assays, where it was tested with primary human NK cells and the NK9.2 cell line as effector cells against CHO cells expressing human CXCR3. The antibody demonstrated cytotoxicity against CXCR3-expressing target cells. Binding studies using a Biacore T200 revealed that 53Hu37 displayed differential binding affinities to human and mouse Fcγ receptors, which are important for ADCC and complement-dependent cytotoxicity (CDC). In vivo, the antibody was administered to cynomolgus monkeys, leading to a reduction in effector memory CD4+ and CD8+ T-cells, as well as CXCR3 staining in the spleen. Biochemical stability analyses showed that 53Hu37 maintained favorable stability under various stress conditions. The antibody also exhibited strong binding kinetics to recombinant human CXCR3 (KD = 0.957 nM) (US20180214542A1).
AB04813-10.0 Datasheet
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