Anti-CD96 [BA143]
Catalogue Number: AB04855-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Tactile; T cell activation, increased late expression; Cluster of Differentiation 96 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | BA143 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing mice with human CD96. |
| Application: | ELISA, FC, FA, InVitroA, Act |
Additional Text
Gene ID
10225
Gene Name
CD96
Uniprot ID
P40200
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original antibody (Mouse IgG1) demonstrated high-affinity binding to CHO cells expressing human CD96 isoform 2 (EC50 = 5.9 ng/mL), as validated in an ELISA. It effectively blocked CD155-Fc binding to the same target (IC50 = 152.4 ng/mL) in a competitive binding assay. It also exhibited binding to CHO cells expressing the V/V variant of human FcγRIIIa (EC50 = 191.5 ng/mL), confirmed through an ELISA. In an in vitro T cell activation assay, the antibody successfully bound to activated CD4+ and CD8+ T cells from three donors, and in a staphylococcal enterotoxin A (SEA)-stimulation assay, it showed limited functional activity in promoting IL-2 secretion by PBMCs. Additionally, it displayed a binding affinity (KD = 13.3 nM) for the full-length extracellular domain of human CD96 in a kinetic analysis (US20240141039A1).
AB04855-1.1-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations