Anti-CD96 [BA143]
Catalogue Number: AB04855-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Tactile; T cell activation, increased late expression; Cluster of Differentiation 96 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | BA143 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing mice with human CD96. |
| Application: | ELISA, FC, FA, InVitroA, Act |
Additional Text
Gene ID
10225
Gene Name
CD96
Uniprot ID
P40200
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
The original antibody (Mouse IgG1) demonstrated high-affinity binding to CHO cells expressing human CD96 isoform 2 (EC50 = 5.9 ng/mL), as validated in an ELISA. It effectively blocked CD155-Fc binding to the same target (IC50 = 152.4 ng/mL) in a competitive binding assay. It also exhibited binding to CHO cells expressing the V/V variant of human FcγRIIIa (EC50 = 191.5 ng/mL), confirmed through an ELISA. In an in vitro T cell activation assay, the antibody successfully bound to activated CD4+ and CD8+ T cells from three donors, and in a staphylococcal enterotoxin A (SEA)-stimulation assay, it showed limited functional activity in promoting IL-2 secretion by PBMCs. Additionally, it displayed a binding affinity (KD = 13.3 nM) for the full-length extracellular domain of human CD96 in a kinetic analysis (US20240141039A1).
AB04855-23.0 Datasheet
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