Anti-ST2L [STLM208 (ST2H257)]
Catalogue Number: AB04870-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | ST2; IL1RL1; IL-1RL1; DER4; T1; IL-33Rα; Interleukin-1 receptor-like 1; Protein ST2; Growth Stimulation Expressed Gene 2; suppression of tumorigenicity 2; ST2F6; ST2F14 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | STLM208 (ST2H257) |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was isolated from a human Fab phage display library by panning against chimeric HHM-ST2L construct with the biotinylated antigen captured on streptavidin-coated magnetic beads. Phage selections were performed for three rounds followed by screening by ELISA for Fab binding to hST2L-Fc protein. The selected Fab was affinity matured and converted to a human IgG1 format. |
| Application: | ELISA, FC, FA, Blk, Ant |
Additional Text
Gene ID
9173
Gene Name
IL1RL1
Uniprot ID
Q01638
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody binds human ST2L and blocks the interaction between IL-33 and ST2L. This antibody was capable of recognizing recombinant human ST2L extracellular domain (ST2L-ECD) using an ELISA assay (PMID: 27294560). The original human IgG2 chimeric version of the antibody binds human and cynomolgus monkey ST2L with a binding affinity of 11 pM and 5 pM respectively (US9090694). Ths antibody inhibited IL-33-induced GM-CSF , IL-5, IL-8, and IL-13 release in primary human lung mast cells at antibody concentrations 100 µg/ml, 10 µg/ml and 1 µg/ml. Similar results were obtained using the cord blood-derived mast cells (US9090694). This antibody can be used for detection of ST2L using flow cytometry.
AB04870-1.1-BT Datasheet
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