Anti-ST2L [STLM208 (ST2H257)]
Catalogue Number: AB04870-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | ST2; IL1RL1; IL-1RL1; DER4; T1; IL-33Rα; Interleukin-1 receptor-like 1; Protein ST2; Growth Stimulation Expressed Gene 2; suppression of tumorigenicity 2; ST2F6; ST2F14 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Human |
| Clone: | STLM208 (ST2H257) |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was isolated from a human Fab phage display library by panning against chimeric HHM-ST2L construct with the biotinylated antigen captured on streptavidin-coated magnetic beads. Phage selections were performed for three rounds followed by screening by ELISA for Fab binding to hST2L-Fc protein. The selected Fab was affinity matured and converted to a human IgG1 format. |
| Application: | ELISA, FC, FA, Blk, Ant |
Additional Text
Gene ID
9173
Gene Name
IL1RL1
Uniprot ID
Q01638
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
This antibody binds human ST2L and blocks the interaction between IL-33 and ST2L. This antibody was capable of recognizing recombinant human ST2L extracellular domain (ST2L-ECD) using an ELISA assay (PMID: 27294560). The original human IgG2 chimeric version of the antibody binds human and cynomolgus monkey ST2L with a binding affinity of 11 pM and 5 pM respectively (US9090694). Ths antibody inhibited IL-33-induced GM-CSF , IL-5, IL-8, and IL-13 release in primary human lung mast cells at antibody concentrations 100 µg/ml, 10 µg/ml and 1 µg/ml. Similar results were obtained using the cord blood-derived mast cells (US9090694). This antibody can be used for detection of ST2L using flow cytometry.
AB04870-10.3 Datasheet
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