Anti-CD20 [HB20-3]
Catalogue Number: AB04887-10.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Bp35; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Human |
| Clone: | HB20-3 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with a mouse pre-B cell line that was transfected with cDNAs encoding human CD20. |
| Application: | IF, Depl |
Additional Text
Gene ID
931
Gene Name
MS4A1
Uniprot ID
P11836
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
The original format of the antibody bound to cell surface CD20, such as human blood lymphocytes and the Raji B lymphoblastoid cell line, by immunofluorescence analysis. The original format of the antibody bound to primary B cells and B lymphoma cell lines expressing CD20 in indirect immunofluorescence staining assays (US9416187B2). The IgG1 and IgG1 YTE formats of the antibody were able to efficiently elicit ADCC with an IC50 of 1.3 +/- 0.5 and 3.7 +/- 1.4 ng/mL, respectively with Daudi as target cells and 4.6 +/- 1.6 and 5.2 +/- 1.7 ng/mL with Raji cells. Further, the IgG1 and IgG1 YTE formats of the antibody were able to potently elicit CDC with IC50 values of 34 and 160 ng/mL, respectively (Borrok et al., 2017; PMID: 28057542).
AB04887-10.0 Datasheet
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