Anti-TNF alpha [hTNF40 (Certolizumab)]

Catalogue Number: AB04906-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	TNF-α; TNFα; TNF; TNFA; TNFSF; Tumor necrosis factor; Cachectin; TNFalpha; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNFa; CDP870; PHA-738144; CIMZIA
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Human
Clone:	hTNF40 (Certolizumab)
Isotype:	IgG1
Immunogen:	The original antibody is a monovalent Fab fragment of a humanized anti-TNFα antibody and lacks the Fc region. Further, the hinge region is attached to two cross-linked chains of a 20-kDa polyethylene glycol (PEG).
Application:	InVitroA, InVivoA, Crstapy

Overview
Datasheets

Additional Text

Gene Name

TNF

Gene ID

7124

Uniprot ID

P01375

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The PEGylated Fab fragment of the antibody has been approved by the FDA as a biologic against TNFα. The original format of the antibody was used for in vitro and in vivo studies, where it showed to neutralize the activity of TNFα. Further, the antibody was effective and well tolerated by patients with RA (Choy et al, 2002; PMID: 12364632). The structure of Fab fragment of the antibody in complex with human TNFα was determined. The binding affinity of the Fab fragment to TNFα was measured by surface plasmon resonance (Kd= 0.2 pM) (Lee et al., 2017; PMID: 28124979). The specificity of the original format of the antibody was confirmed by ELISA analysis (US7012135). The original format of the antibody bound and neutralised both soluble and membrane-bound forms of TNFα. The pharmacokinetic properties of the PEGylate Fab fragments in vivo resulted in a half-life of 14 days. Isothermal titration calorimetry data showed that the original format of the antibody bound to 2.9 monomers in a TNFα trimer at saturation. The antibody was employed in in vivo studies in rats, primates and humans. Several clinical trials have also been conducted to examine the efficacy and tolerability of the original format of the antibody in patients with CD, RA and psoriasis (Filipovica et al., 2009).

AB04906-10.0 Datasheet

Price

£430.00

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