Anti-CTLA-4 [10D1 (Ipilimumab)]

Catalogue Number: AB04910-3.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CTLA4; CD152; MDX-010; MDX-CTLA-4; MDX-CTLA4; YERVOY; BCD-145; nurulimab; BMS-734016; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	10D1 (Ipilimumab)
Isotype:	IgG2b
Immunogen:	The original antibody was generated by immunizing transgenic mice with human CTLA-4.
Application:	ELISA, FACS, FC, WB, Inh, InVitroA, InVivoA, SPR, Crstapy

Overview
Datasheets

Additional Text

Uniprot ID

P16410

Gene ID

1493

Gene Name

CTLA4

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding affinity of the IgG1 fragment to CTLA-4 was measured by surface plasmon resonance (Kd= 4 nM). This antibody bound to CTLA-4 expressed on the plasma membrane of T-cells and CTLA-4 expressed by normal human T cells by flow cytometry. The original format of the antibody stained positive control, human CTLA-4-expressing, 58αβCTLA4CD3zeta cells as well as positive control lymphocytes in human tonsil using an indirect immunoperoxidase method. The original format of the antibody reacted with macaque and human CTLA-4, but not with murine CTLA-4 by FACS analysis. The original format of the antibody efficiently inhibited CTLA-4-B7 interactions at low concentrations (1-10 µg/ml) by flow cytometry (US6984720B1). The original format of the antibody was shown to mediate antibody-dependent cell-mediated cytotoxicity (ADCC)-facilitated depletion of Treg cells in vitro (Romano et al., 2015; PMID: 25918390). The crystal structure of the Fab fragment of the antibody in complex with human CTLA-4 was determined. The Fab fragment of the antibody competed with hB7-1 for binding to hCTLA-4 in a bead-based FACS assay. The Fab fragment of the antibody detected human CTLA-4 by western blot analysis (Ramagopal et al., 2017; PMID: 28484017). The antibody received FDA approval for the treatment of metastatic melanoma in 2011.

AB04910-3.0 Datasheet

Price

£430.00

-

Quantity
Unit Size

Find out about delivery