Anti-S1P [LT1009]
Catalogue Number: AB05044-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Sonepcizumab; sphingosine-1-phosphate; lysosphingolipid |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | LT1009 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with S1P-SMCC-KLH followed by humanization by grafting the CDR loops of the murine parent antibody onto a human IgG1K isotype framework. |
| Application: | ELISA, InVivoA, SPR, Crstapy |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
The specificity of the original format of the antibody for S1P was confirmed by ELISA analysis. The crystal structure of the Fab fragment of the antibody in complex with S1P was determined. The Fab format of the antibody bound S1P with an apparent equilibrium dissociation binding constant (KD) of 130 nM, as measured by SPR (Wojciak et al., 2009; PMID: 19815502). The original format of the antibody exhibited greater reduction of IL-8 release in SKOV3 tumor cells. The original format of the antibody suppressed neovascularization in in vivo experiments. The original format of the antibody retarded the progression of human colorectal (COLO0205) carcinoma tumors, human prostate (DU145) carcinoma tumors and human HT29 colon tumor xenografts implanted in mice. The combination of the original format of the antibody and Avastin was more effective than either agent alone for COLO0205 and HT29 tumors. Finally, the original format of the antibody reduced the weight of human myeloma tumor xenografts implanted in mice alone or together with Bortezomib (US8067549B2).
AB05044-10.0-BT Datasheet
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