Anti-C3D [3d8b], Mouse IgG2b, kappa
Catalogue Number: AB05050-3.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | C3; CPAMD1; Complement C3; Complement C3d fragment; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 3d8b |
| Isotype: | IgG2b |
| Immunogen: | This antibody was raised by immunizing a mouse with a targeted deletion of the C3 gene with human C3d. |
| Application: | ELISA, IF, WB, InVitroA, InVivoA, SPR |
Additional Text
Gene Name
C3
Gene ID
718
Uniprot ID
P01024
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody was used for an ELISA on human C3d. This antibody was used for a western blot on human C3d, it showed strong binding. The affinity of this antibody to C3d was determined using surface plasmon resonance, it showed strong binding with a Kds of 0.47 nM. This antibody also showed it could inhibit the CR2-C3d interaction in a competition ELISA. This antibody was used in vivo on mice, it was shown that this antibody could detect tissue- bound C3 fragments, it readily detected C3 along the glomerular basement membrane (Thurman et al., 2013; PMID:23619360). The structure of this antibody was determined using x-ray crystallography. A fusion protein of this antibody with the first 10 consensus repeats of the human CR1 protein was used in vitro for a Wieslab assay, a hemolysis assay and a HMEC assay. The pharmacokinetics (PK) in mice were determined for various fusion proteins containing this antibody (Fahnoe et al., 2022; PMID:35784298). A fusion protein of this antibody with factor H was used for a complement inhibition assay it showed an IC50 of 73 nM. This antibody was used for immunofluorescence on human skin biopsies and kidney tissues (Liu et al., 2024; PMID:38382529).
AB05050-3.0 Datasheet
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