Anti-Aquaporin 4 [rAb-53 (affinity matured)], Mouse IgG2b, kappa

Catalogue Number: AB05080-3.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	AQP4; AQP-4; AQmab; aquaporumab; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel; rAb53; Aquaporumab; AQmabAM
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Mouse
Clone:	rAb-53 (affinity matured)
Isotype:	IgG2b
Immunogen:	The original antibody rAb-53 was generated from the PCR products obtained from the human B cell clones isolated from CSF plasma of an neuromyelitis optica (NMO-IgG) seropositive patient using a dual vector transient transfection system. The affinity matured version was generated by using saturation mutagenesis.
Application:	ELISA, IF, FA

Overview
Datasheets

Additional Text

Gene Name

AQP4

Uniprot ID

P55087

Gene ID

361

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

This antibody is an affinity matured version of rAb-53 (Aquaporumab). The binding of this antibody to the extracellular region of AQP4 in CHO cells stably expressing the M23 isoform of human AQP4 was done using a cell-based ELISA. When AQP4-expressing cells were incubated with affinity matured rAb-53 and human complement antibody it produced complement-dependent cytotoxicity (CDC) with EC50 ~ 82 ng/ml. The Fc silent version of this antibody with LALA mutations, also called as AQmabAM bound to AQP4 in cell cultures with Kd ~ 18 ng/ml (~ 0.12 nM). AQmabAM prevented CDC produced by sera from eight NMO patients with IC50 ranging from 40-80 ng/ml, and similarly prevented antibody-dependent cellular cytotoxicity (ADCC). Mechanistic studies demonstrated that AQmabAM blocked binding of serum NMO autoantibodies to AQP4. This antibody can be used for the visualization of AQP4 on the surface of cells using immunofluorescence (PMID: 31654702).

AB05080-3.0-BT Datasheet

Price

£1,825.00

-

Quantity
Unit Size

Find out about delivery