Anti-Aquaporin 4 [rAb58], Mouse IgG2b, kappa

Catalogue Number: AB05081-3.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	AQP4; AQP-4; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	rAb58
Isotype:	IgG2b
Immunogen:	The original antibody was generated from the PCR products obtained from the human B cell clones isolated from CSF plasma of an neuromyelitis optica (NMO-IgG) seropositive patient using a dual vector transient transfection system.
Application:	FC, IF, FA

Overview
Datasheets

Additional Text

Gene Name

AQP4

Uniprot ID

P55087

Gene ID

361

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

This antibody was reported to mediate AQP4-directed antibody-dependent cellular cytotoxicity and complement-mediated lysis in vitro. The binding characterization of this antibody to AQP4 expressed on Human Fetal Astrocyte (HFA) was done by quantitative flow cytometry (PMID: 19938104). Binding of rAb-58 to the M1 and M23 AQP4 isoforms was measured by the fluorescence ratio imaging method (PMID: 21454592). CHO cells expressing M1- or M23-AQP4 when labeled with rAb-58 conjugated to the red fluorophore Cy3 (rAb-58-Cy3), showed rapid internalization of M23-AQP4 than M1-AQP4 when expressed separately in transfected cells. When both isoforms were co-expressed in OAPs in transfected cells, the antibody causes simultaneous cointernalization of M1- and M23-AQP4 isoforms (PMID: 22987455).

AB05081-3.0 Datasheet

Price

£430.00

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