Anti-Aquaporin 4 [rAb58], Mouse IgG2b, Fc Silent™, kappa
Catalogue Number: AB05081-3.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | AQP4; AQP-4; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | rAb58 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated from the PCR products obtained from the human B cell clones isolated from CSF plasma of an neuromyelitis optica (NMO-IgG) seropositive patient using a dual vector transient transfection system. |
| Application: | FC, IF, FA |
Additional Text
Gene Name
AQP4
Uniprot ID
P55087
Gene ID
361
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Short Description
This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Application Notes
This antibody was reported to mediate AQP4-directed antibody-dependent cellular cytotoxicity and complement-mediated lysis in vitro. The binding characterization of this antibody to AQP4 expressed on Human Fetal Astrocyte (HFA) was done by quantitative flow cytometry (PMID: 19938104). Binding of rAb-58 to the M1 and M23 AQP4 isoforms was measured by the fluorescence ratio imaging method (PMID: 21454592). CHO cells expressing M1- or M23-AQP4 when labeled with rAb-58 conjugated to the red fluorophore Cy3 (rAb-58-Cy3), showed rapid internalization of M23-AQP4 than M1-AQP4 when expressed separately in transfected cells. When both isoforms were co-expressed in OAPs in transfected cells, the antibody causes simultaneous cointernalization of M1- and M23-AQP4 isoforms (PMID: 22987455).
AB05081-3.3-BT Datasheet
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