Anti-uPAR [2.6.1], Mouse IgG2b, Fc Silent™, kappa

Catalogue Number: AB05099-3.3-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CD87; U-PAR; MO3; PLAUR; Urokinase plasminogen activator surface receptor; Monocyte activation antigen Mo3
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	2.6.1
Isotype:	IgG2b
Immunogen:	The original antibody was generated by immunizing XenoMouse® with human uPAR expressed on the surface of B cells. Recovered lymphocytes from hyperimmunized mice are fused with a myeloid-type cell line to prepare immortal hybridoma cell lines. Antibodies from the cell supernatants are then screened by ELISA for reactivity against the uPAR immunogen.
Application:	FC, FA, Inh

Overview
Datasheets

Additional Text

Uniprot ID

Q03405

Gene Name

PLAUR

Gene ID

5329

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody inhibits uPA binding to the cell surface of U937 cells. In an adhesion assay, this antibody inhibited cell adhesion of U937 cells to vitronectin in a dose-dependent manner. At a concentration of 2 µg/ml, 2.6.1 achieved 90% of inhibition. The binding characterization of this antibody to human UPAR was done using High Resolution Biacore™. The binding affinity of the original IgG isotype for human UPAR was reported to be Kd= 249pM. The binding characterization of biotin conjugated version of this antibody was done using immunohistochemistry on frozen cell pellets and normal human tissue microarrays (US8026344).

Short Description

This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG2 format, created for improved compatibility with existing reagents, assays and techniques.

AB05099-3.3 Datasheet

Price

£430.00

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