Anti-LCMV surface glycoprotein [M28], Human Fab fragment, His-Tagged, kappa

Catalogue Number: AB05114-10.29-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	LCMV surface GP; lineage I Lassa fever virus; lineage I LASV; LASV; Pre-glycoprotein polyprotein GP complex; Pre-GP-C; Stable signal peptide; SSP; Glycoprotein G1; GP1; Glycoprotein G2; GP2; 18.5C-M28
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Human
Clone:	M28
Isotype:
Immunogen:	The original antibody was generated by introducing two mutations (S32R and R55 insertion) into the sequence of the parent clone 18.5C (an isolate from a human survivor of Lassa fever).
Application:	ELISA, EM, NT, InVitroA, InVivoA

Overview
Datasheets

Additional Text

Purification

Purified

UniProt ID

P07399

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original antibody (human IgG1) was generated and confirmed to specifically recognize the target via ELISA. A Fab version of it was generated and tested for neutralization. This antibody demonstrated potent in vitro neutralization (IC₅₀ ~ 0.51 µg/mL) and both prophylactic and therapeutic efficacy in a murine chronic LCMV model, with complete viral clearance and protection from weight loss. Furthermore, this antibody enabled high-resolution cryoEM of the LCMV GP trimer in both apo and Fab-bound states, defining a quaternary, pre-fusion-specific epitope (Moon-Walker et al., 2023; PMID: 36990092). In vitro, this antibody fully neutralized cell-free LCMV at 5 µg/mL and was used to demonstrate that ~50% of viral spread occurs via antibody-resistant, direct cell-to-cell nanotube transfer (Byford et al., 2024; PMID: 39578605).

AB05114-10.29 Datasheet

Price

£525.00

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