Anti-Capsular polysaccharide [2-2-B (5C1-3H7)], Mouse IgM, kappa

Catalogue Number: AB05118-21.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Capsular PS; type B capsular PS; serogroup B sialic acid containing capsular polysaccharides; MBPS
Shipping Condition:	Blue Ice
Unit(s):	50 ug
Host name:	Mouse
Clone:	2-2-B (5C1-3H7)
Isotype:	IgM
Immunogen:	The original antibody was generated by immunizing mice with whole encapsulated N. Meningitidis.
Application:	ELISA, FC, ImGldLbl

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The original antibody format (Mouse IgM) was generated and evaluated. Its specificity was determined by indirect whole-cell and purified polysaccharide ELISA. It exhibited strong binding to serogroup B capsular polysaccharide (OD₄₀₅ ~ 0.85 on strain 99M) with only background-level reactivity to serogroup C and the other 12 serogroups, demonstrating exquisite type B specificity (Berry et al., 2005; PMID: 15589322). This antibody was successfully used to investigate capsule expression in Neisseria meningitidis serogroup B. It was used in a whole-cell ELISA to quantify total capsular polysaccharide production, in flow cytometry to detect surface and intracellular capsular polysaccharide, and in immunogold electron microscopy to visualize capsular polysaccharide localization (Tzeng et al., 2005; PMID: 15731047). This antibody underwent genetic analysis to reveal its unmutated V genes, structural modeling to identify a shallow combining site groove, and used as a control for autoreactivity to confirm strong PSA binding, contrasting with non-autoreactive anti-N-Pr MBPS mAbs (Moe et al., 2006; PMID: 16140379).

AB05118-21.0 Datasheet

Price

£525.00

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