Anti-Capsular polysaccharide [C2/655.7], Mouse IgG1, kappa
Catalogue Number: AB05119-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Capsular PS; meningococcal polysaccharide group C; serogroup C sialic acid containing capsular polysaccharides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | C2/655.7 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing female BALB/c mice with a group C N. Meningitidis (strain C11) oligosaccharide coupled to tetanus toxoid (MCPS-TT). |
| Application: | ELISA, WB, FLISA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original antibody format (Mouse IgG1) was generated and evaluated. Its specificity was determined by FELISA. It exhibited a binding pattern of OAc- >> MCPS, with 50% binding observed at approximately 0.003 µg/mL and 0.3 µg/mL, respectively, under the assay conditions. This indicates a strong preference for the de-O-acetylated serogroup C capsular polysaccharide. The original antibody's pI was measured to be in the range of 5.6-6.7 (Garcia-Ojeda et al., 2000; PMID: 10603394). Surface plasmon resonance analysis confirmed its higher avidity and preference for the OAc- surface, although kinetic studies revealed short dissociation half-lives for both OAc+ and OAc- surfaces (Garcia-Ojeda, 2004; PMID: 15155652). This antibody was successfully used in a WB to detect OAc- (Mosley et al., 2009; DOI: 10.3109/10242420903388694).
AB05119-1.1-BT Datasheet
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