Anti-Capsular polysaccharide [C2/655.7], Rabbit IgG, kappa

Catalogue Number: AB05119-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Capsular PS; meningococcal polysaccharide group C; serogroup C sialic acid containing capsular polysaccharides
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Rabbit
Clone:	C2/655.7
Isotype:	IgG
Immunogen:	The original antibody was generated by immunizing female BALB/c mice with a group C N. Meningitidis (strain C11) oligosaccharide coupled to tetanus toxoid (MCPS-TT).
Application:	ELISA, WB, FLISA

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original antibody format (Mouse IgG1) was generated and evaluated. Its specificity was determined by FELISA. It exhibited a binding pattern of OAc- >> MCPS, with 50% binding observed at approximately 0.003 µg/mL and 0.3 µg/mL, respectively, under the assay conditions. This indicates a strong preference for the de-O-acetylated serogroup C capsular polysaccharide. The original antibody's pI was measured to be in the range of 5.6-6.7 (Garcia-Ojeda et al., 2000; PMID: 10603394). Surface plasmon resonance analysis confirmed its higher avidity and preference for the OAc- surface, although kinetic studies revealed short dissociation half-lives for both OAc+ and OAc- surfaces (Garcia-Ojeda, 2004; PMID: 15155652). This antibody was successfully used in a WB to detect OAc- (Mosley et al., 2009; DOI: 10.3109/10242420903388694).

AB05119-23.0 Datasheet

Price

£430.00

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