Anti-Capsular polysaccharide [2016.3], Mouse IgG1, kappa
Catalogue Number: AB05121-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Capsular PS; meningococcal polysaccharide group C; serogroup C sialic acid containing capsular polysaccharides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 2016.3 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing female BALB/c mice with native, unconjugated group C N. Meningitidis (strain C11) capsular polysaccharide (MCPS). |
| Application: | ELISA, FLISA, Inh |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Short Description
This reformatted mouse antibody was made using the variable domain sequences of the original mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
Application Notes
The original antibody format (Mouse IgG3) was generated and evaluated. Its specificity was determined by FELISA. It exhibited a binding pattern of OAc- >> MCPS, with 50% binding observed at approximately 0.1 µg/mL and >3 µg/mL, respectively, under the assay conditions. This indicates a strong preference for the de-O-acetylated serogroup C capsular polysaccharide. The original antibody's pI was measured to be in the range of 8.3-8.6 (Garcia-Ojeda et al., 2000; PMID: 10603394). Surface plasmon resonance analysis indicated lower avidity with a preference for the OAc- surface, as shown by greater concentration-dependent binding to OAc-; however, kinetic studies did not reflect this preference in dissociation half-lives. Furthermore, in inhibition experiments, it partially blocked the binding of conjugate vaccine immune sera to OAc- surfaces, achieving less than 40% inhibition (Garcia-Ojeda, 2004; PMID: 15155652).
AB05121-1.1-BT Datasheet
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