Anti-Capsular polysaccharide [2016.3], Mouse IgG3, Fc Silent™, kappa
Catalogue Number: AB05121-5.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Capsular PS; meningococcal polysaccharide group C; serogroup C sialic acid containing capsular polysaccharides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 2016.3 |
| Isotype: | IgG3 |
| Immunogen: | The original antibody was generated by immunizing female BALB/c mice with native, unconjugated group C N. Meningitidis (strain C11) capsular polysaccharide (MCPS). |
| Application: | ELISA, FLISA, Inh |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original antibody format (Mouse IgG3) was generated and evaluated. Its specificity was determined by FELISA. It exhibited a binding pattern of OAc- >> MCPS, with 50% binding observed at approximately 0.1 µg/mL and >3 µg/mL, respectively, under the assay conditions. This indicates a strong preference for the de-O-acetylated serogroup C capsular polysaccharide. The original antibody's pI was measured to be in the range of 8.3-8.6 (Garcia-Ojeda et al., 2000; PMID: 10603394). Surface plasmon resonance analysis indicated lower avidity with a preference for the OAc- surface, as shown by greater concentration-dependent binding to OAc-; however, kinetic studies did not reflect this preference in dissociation half-lives. Furthermore, in inhibition experiments, it partially blocked the binding of conjugate vaccine immune sera to OAc- surfaces, achieving less than 40% inhibition (Garcia-Ojeda, 2004; PMID: 15155652).
Short Description
This is a reformatted mouse IgG3 Fc Silent™ antibody, based on the original mouse IgG3 format, created for improved compatibility with existing reagents, assays and techniques.
AB05121-5.3-BT Datasheet
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