Anti-Chlamydia lipopolysaccharide [S25-23], Mouse IgG1, kappa

Catalogue Number: AB05126-1.1-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:LPS; Kdo(2 to 8)Kdo(2 to 4)Kdo
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: S25-23
Isotype: IgG1
Immunogen: This antibody was raised by immunizing a BALB/c mouse with KD03-GlcNAc-BSA.
Application: ELISA, WB, FA

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

Multiple different formats of this antibody were used for surface plasmon resonance on alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo, it showed an affinity between 4.5 * 10^4 M^-1 * s^-1 and 5.9 * 10^4 M^-1 * s^-1 (Mueller-Loennies et al., 2000; PMID:10642603). This antibody was used for an ELISA on KDO3-GIcNAc-BSA to characterize this antibody. This antibody was used for a western blot on chlamydia LPS, it showed it was able to bind to r595-207 LPS (Fu et al., 1992; PMID:1372290, Haji-Ghassemi et al., 2018; PMID:30571096, Brade et al., 1997; PMID:9376684). The structure of this antibody was determined using x-ray crystallography (Haji-Ghassemi et al., 2018; PMID:30571096).